Coding

Part:BBa_K4940001:Design

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-10)


P450BM-3 QM-his


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1373
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1373
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1373
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1219


Design Notes

In our project, we only found the common sequence of P450 in this organism from the NCBI database. So we manually introduced these five mutations to the DNA sequence. At the same time, his tag was fused to the protein, for better protein purification.

Source

P450BM-3 QM is a mutant type of common P450, and it is first extracted from the genome of Bacillus megaterium. In our project, we found the common sequence of P450 in this organism from the NCBI database and manually introduced these five mutations to the DNA sequence.

References